Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata

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dc.contributor.authorTomičić, Zorica
dc.contributor.authorZupan Jure
dc.contributor.authorMatos, Tadeja
dc.contributor.authorRaspor, Peter
dc.date.accessioned2017-11-21T16:51:54Z
dc.date.available2017-11-21T16:51:54Z
dc.date.issued2016-11-01
dc.descriptionpeer-revieweden_US
dc.description.abstractFollowing the widespread use of immunosuppressive therapy together with broad-spectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by the pathogenic yeast Candida glabrata has increased in the past decades. Due to the resistance of C. glabrata to existing azole drugs, it is very important to look for new strategies helping the treatment of such fungal diseases. In this study, we investigated the effect of the probiotic yeast Saccharomyces boulardii (nom. nud.) on C. glabrata adhesion at different temperatures, pH values, and in the presence of fluconazole, itraconazole and amphotericin B. We also studied the adhesion of C. glabrata co-culture with Candida krusei, Saccharomyces cerevisiae, two bacterial probiotics Lactobacillus rhamnosus and Lactobacillus casei The method used to assess adhesion was crystal violet staining. Our results showed that despite the nonadhesiveness of S. boulardii cells, this probiotic significantly affected the adherence ability of C. glabrata This effect was highly dependent on C. glabrata strain and was either antagonistic or synergistic. Regarding the extrinsic factors, temperature did not indicate any significant influence on this S. boulardii modulatory effect, while at high pH and at increased concentrations of antimycotics, S. boulardii did not manage to repress the adhesion of C. glabrata strains. The experiments of C. glabrata co-cultures with other species showed that the adhesiveness of two separate cultures could not be used to predict the adhesiveness of their co-culture.en_US
dc.description.sponsorshipWe thank Neža Čadež, the curator of the Collection of Industrial Microorganisms (ZIM) at the Biotechnical Faculty, University of Ljubljana, Slovenia, for providing us with the yeast strains. Z. T. thank the Ministry of Education, Science and Technological Development Republic of Serbia (project no. III46012) for financial support during study stay at the Biotechnical faculty in Ljubljana. We also thank Ruzica Tomičić, the researcher at the Faculty of Technology, University of Novi Sad, Serbia, for her help with the crystal violet staining protocol. The experimental work and English correction of this manuscript were financially supported by the Slovenian Ministry of Higher Education, Science and Technology (grant no. Z7-4248).en_US
dc.identifier.citationTomičić, Z., Zupan, J., Matos, T., Raspor, P. (2016) Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata. Medical Mycology, 54, 835–845. DOI: 0.1093/mmy/myw026en_US
dc.identifier.issn1369-3786
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/31
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46012/RS//
dc.relation.ispartofseries001;0026
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcandida glabrataen_US
dc.subjectsaccharomyces boulardiien_US
dc.subjectadhesionen_US
dc.subjectantimycoticsen_US
dc.subjectcell surface hydrophobicityen_US
dc.titleProbiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrataen_US
dc.title.alternative-en_US
dc.typeinfo:eu-repo/semantics/articleen_US

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