Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata
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Date
2016-11-01
Authors
Tomičić, Zorica 
Zupan Jure
Matos, Tadeja
Raspor, Peter
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Abstract
Following the widespread use of immunosuppressive therapy together with broad-spectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by the pathogenic yeast Candida glabrata has increased in the past decades. Due to the resistance of C. glabrata to existing azole drugs, it is very important to look for new strategies helping the treatment of such fungal diseases. In this study, we investigated the effect of the probiotic yeast Saccharomyces boulardii (nom. nud.) on C. glabrata adhesion at different temperatures, pH values, and in the presence of fluconazole, itraconazole and amphotericin B. We also studied the adhesion of C. glabrata co-culture with Candida krusei, Saccharomyces cerevisiae, two bacterial probiotics Lactobacillus rhamnosus and Lactobacillus casei The method used to assess adhesion was crystal violet staining. Our results showed that despite the nonadhesiveness of S. boulardii cells, this probiotic significantly affected the adherence ability of C. glabrata This effect was highly dependent on C. glabrata strain and was either antagonistic or synergistic. Regarding the extrinsic factors, temperature did not indicate any significant influence on this S. boulardii modulatory effect, while at high pH and at increased concentrations of antimycotics, S. boulardii did not manage to repress the adhesion of C. glabrata strains. The experiments of C. glabrata co-cultures with other species showed that the adhesiveness of two separate cultures could not be used to predict the adhesiveness of their co-culture.
Description
peer-reviewed
Keywords
candida glabrata, saccharomyces boulardii, adhesion, antimycotics, cell surface hydrophobicity
Citation
Tomičić, Z., Zupan, J., Matos, T., Raspor, P. (2016) Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata. Medical Mycology, 54, 835–845. DOI: 0.1093/mmy/myw026