Eco-Friendly Extraction: A green approach to maximizing bioactive extraction from pumpkin (Curcubita moschata L.)
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Date
2024-03-23
Authors
Matić, Milana 
Stupar, Alena
Pezo, Lato
Đerić, Nataša
Teslić, Nemanja
Pojić, Milica
Mandić, Anamarija
Mišan, Aleksandra
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Ltd
Abstract
The research focused on optimizing the accelerated solvent extraction (ASE) of carotenoids and polyphenols from pumpkin powder. The study optimized accelerated solvent extraction (ASE) of carotenoids and polyphenols from pumpkin powder. Using a mix of standard score (SS) and artificial neural network (ANN) methods, the extraction process was fine-tuned. The ANN model assessed extraction parameters' significance, achieving high predictability for total carotenoid content (TCC), total phenolic content (TPC), and free radical scavenging capacity (DPPH and ABTS methods). The analysis highlighted the most effective extraction at 50 % concentration, 120 °C temperature, 5 min duration, and 2 cycles, yielding high carotenoid and phenolic content (TCC 571.49 µg/g, TPC 7.85 mg GAE/g). HPLC-DAD profiles of the optimized ASE extract confirmed major carotenoids and phenolic compounds. Strong correlations were found between bioactive compounds and antioxidant activity, emphasizing potential health benefits.
Description
Keywords
SE optimization, Pumpkin, Carotenoids, Polyphenols, Antioxidant activity, Artificial neural network (ANN)
Citation
Matić, M., Stupar, A., Pezo, L., Ilić, N. Đ., Mišan, A., Teslić, N., ... & Mandić, A. (2024). Eco-Friendly Extraction: A green approach to maximizing bioactive extraction from pumpkin (Curcubita moschata L.). Food Chemistry: X, 22, 101290.