Institute of Food Technology

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Browsing Institute of Food Technology by Subject "adhesion"

Now showing 1 - 6 of 6
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    Adhesion of bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and yeast Pichia membranifaciens to wooden surfaces
    (European Declaration on Food, Technology and Nutrition Network, Ljubljana, 2022, 2022-09-27) Tomičić, Ružica; Tomičić, Zorica; Thaler, Nejc; Humar, Miha; Raspor, Peter
    Microbial adhesion and biofilm formation on wooden surfaces is present in many different environments. In the food industry, biofilms can be a source of contaminations, causing food spoilage and reducing quality of products. Therefore, the aim of this study was to evaluate the potential of bacteria Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and yeast Pichia membranifaciens ZIM 2417 to adhere to wooden surfaces such as poplar (Populus sp.), Norway spruce (Picea abies), European beech (Fagus sylvatica) as well as European beech coated with commercial Belinka oil food contact and disinfectant P3-oxonia active 150. In order to better define the factors that could influence the adhesion of bacteria and yeast to European beech surfaces, temperature (10°C, 20°C, 27°C/37°C) and relative humidity (RH; 65%, 75%, 85%, 98%) were also examined. Adhesion was determined by the number of colony-forming units per mm2 of sample (CFU/mm2). The results showed that gram-negative bacteria E. coli and P. aeruginosa exhibited a much better ability for adherence to wooden surfaces than gram-positive bacteria S. aureus and yeast P. membranifaciens. It was evident that adhesion of S. aureus and P. membranifaciens was lower on spruce compared to poplar and beech, indicating that this wooden species possesses substantially better hygienic characteristics. In addition, noteworthy is the fact that a drastic decrease in the number of adhered cells for E. coli, S. aureus and P. membranifaciens became apparent when the beech wood surfaces were coated with oil and disinfectant, while in the case of P. aeruginosa adhesion was significantly stimulated. Our data also indicated that the lowest relative humidity and temperature had a repressive effects on the adherence of all tested bacteria and yeast, suggesting that these findings offers a great potential applicability in food-processing industry for the purpose of controlling the possibility of biofilm formation.
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    Adhesion of Candida spp. and Pichia spp. to wooden surfaces
    (Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2017-03) Tomičić, Ružica; Tomičić, Zorica; Raspor, Peter
    Yeast adhesion to and biofilm formation on surfaces is present in many different environments. In food industry, biofilms may be a source of contaminations, causing food spoilage and reducing quality of products. Candida and Pichia are two common yeast genera involved in the spoilage of some food products. The aim of this study is to assess the potential of Candida and Pichia species to adhere to two types of wooden surfaces (smooth and rough), one of the materials typical for the food processing industry, and investigate the influence of surface roughness of wood on the degree of yeast adhesion. The adhesion of the cells to the wooden surfaces was determined by rinsing them from the surface, followed by methylene blue staining, and quantification after imaging under microscope by automatic counting of viable cells. The results showed that all Candida and Pichia strains were able to adhere to the wooden surfaces in a species- and strain-dependent manner. On the other hand, our data indicated that adhesion by these yeasts was not significantly affected by the roughness of the wood surfaces.
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    The effect of plant extracts and essential oils on the adhesion of Candida glabrata
    (European Declaration on Food, Technology and Nutrition Network, Ljubljana, 2022, 2022-09-27) Tomičić, Zorica; Tomičić, Ružica; Smole Možina, Sonja; Bucar, Franz; Turek, Ivana; Raspor, Peter
    The incidence of fungal infections, particularly those caused by Candida species (candidiasis) has increased significantly, causing high levels of morbidity and mortality. This fact is mainly due to the rise in antimicrobial resistance and the limited number of efficient antifungal drugs, which still have many side effects. The use of natural antimicrobial agents has gained much attention to inhibit disease-causing microorganisms. Therefore, the aim of this study was to evaluate the antifungal and anti-adhesion potential of fifteen plant extract, essential oils and compounds against Candida glabrata. Susceptibility tests indicated that essential oils of Cinnamomum verum and Origanum vulgare showed the highest inhibitory effect. Anti-adhesion ability of the plant extracts and essential oils against C. glabrata ZIM 2369 was estimated by the standard crystal violet assay. The results showed that the essential oils of C. verum, O.vulgare, Satureja montana and Thymus vulgaris have promising activity against the initial phase of biofilm formation and the preformed 24h biofilm. In contrast, the essential oils of Salvia officinalis and Salvia sclarea were inactive against C. glabrata biofilm. On the other hand, Sedum roseum extract showed the strongest antiadhesion effect. As Candida biofilms are intrinsically resistant to conventional antifungal agents, alternative therapeutics like plant-based natural products have been evaluated for their antimicrobial and anti-biofilm activity. Understanding more about the antimicrobial performance and possible mechanism of the tested plant extracts and essential oils will be helpful for their application against Candida spp. in medicine in the future.
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    Microbial survival on different wooden surfaces
    (University PIM, Banja Luka, Republic of Srpska, B&H, 2023-06-15) Tomičić, Ružica; Nićetin, Milica; Filipović, Vladimir; Lončar, Biljana; Knežević, Violeta; Tomičić, Zorica
    Wood has a long tradition as a natural material used by humans in the preparation, packaging and transport of food products. However, microbial adhesion and subsequent biofilm formation on wooden surfaces can be a source of contaminations that may seriously affect the safety and quality of foods. Hence this study aimed to assess the adhesion of bacteria Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and yeast Pichia membranifaciens ZIM 2417 on different types of wooden surfaces such as poplar (Populus sp.), Norway spruce (Picea abies), European beech (Fagus sylvatica), and to evaluate whether Belinka oil food contact and disinfectant P3-oxonia active 150 can interfere with adhesion to beech surfaces. The adhesion was determined by the number of colony-forming units per mm2 of sample (CFU/mm2). The results showed that bacteria cells noticeably had a higher ability to adhere to wood in comparison with yeast. Evaluation of adhesion revealed that the gram-negative bacteria E. coli ATCC 35218 and P. aeruginosa ATCC 27853 exhibited a much better ability for adherence to wooden surfaces than gram-positive bacteria S. aureus ATCC 25923. In addition, it should be pointed out that wood species like Norway spruce had antimicrobial effect against S. aureus ATCC 25923 and P. membranifaciens ZIM 2417 compared to poplar and European beech. Agents used in the food industry to maintain food contact surfaces such as the Belinka oil food contact and disinfectant P3-oxonia active 150 also showed antimicrobial properties and consequently lower adhesion of microbes to beech surfaces.
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    Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata
    (Oxford University Press, 2016-11-01) Tomičić, Zorica; Zupan Jure; Matos, Tadeja; Raspor, Peter
    Following the widespread use of immunosuppressive therapy together with broad-spectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by the pathogenic yeast Candida glabrata has increased in the past decades. Due to the resistance of C. glabrata to existing azole drugs, it is very important to look for new strategies helping the treatment of such fungal diseases. In this study, we investigated the effect of the probiotic yeast Saccharomyces boulardii (nom. nud.) on C. glabrata adhesion at different temperatures, pH values, and in the presence of fluconazole, itraconazole and amphotericin B. We also studied the adhesion of C. glabrata co-culture with Candida krusei, Saccharomyces cerevisiae, two bacterial probiotics Lactobacillus rhamnosus and Lactobacillus casei The method used to assess adhesion was crystal violet staining. Our results showed that despite the nonadhesiveness of S. boulardii cells, this probiotic significantly affected the adherence ability of C. glabrata This effect was highly dependent on C. glabrata strain and was either antagonistic or synergistic. Regarding the extrinsic factors, temperature did not indicate any significant influence on this S. boulardii modulatory effect, while at high pH and at increased concentrations of antimycotics, S. boulardii did not manage to repress the adhesion of C. glabrata strains. The experiments of C. glabrata co-cultures with other species showed that the adhesiveness of two separate cultures could not be used to predict the adhesiveness of their co-culture.
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    Probiotic yeast Saccharomyces boulardii (nom. nud.) modulates adhesive properties of Candida glabrata
    (Medical Mycology, 2016-06-01) Tomičić, Zorica; Zupan, Jure; Matos, Tadeja; Raspor, Peter
    Following the widespread use of immunosuppressive therapy together with broadspectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by the pathogenic yeast Candida glabrata has increased in the past decades. Due to the resistance of C. glabrata to existing azole drugs, it is very important to look for new strategies helping the treatment of such fungal diseases. In this study, we investigated the effect of the probiotic yeast Saccharomyces boulardii (nom. nud.) on C. glabrata adhesion at different temperatures, pH values, and in the presence of fluconazole, itraconazole and amphotericin B.We also studied the adhesion of C. glabrata co-culture with Candida krusei, Saccharomyces cerevisiae, two bacterial probiotics Lactobacillus rhamnosus and Lactobacillus casei. The method used to assess adhesion was crystal violet staining. Our results showed that despite the nonadhesiveness of S. boulardii cells, this probiotic significantly affected the adherence ability of C. glabrata. This effect was highly dependent on C. glabrata strain and was either antagonistic or synergistic. Regarding the extrinsic factors, temperature did not indicate any significant influence on this S. boulardii modulatory effect, while at high pH and at increased concentrations of antimycotics, S. boulardii did not manage to repress the adhesion of C. glabrata strains. The experiments of C. glabrata co-cultures with other species showed that the adhesiveness of two separate cultures could not be used to predict the adhesiveness of their co-culture.