Isolation of Garlic Bioactives by Pressurized Liquid and SubcriticalWater Extraction

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Date

2023-01-02

Authors

Krstić, Marko
Teslić, Nemanja orcid-logo
Bošković, Perica
Obradović, Darija
Zeković, Zoran
Milić, Anita
Pavlić, Branimir

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI

Abstract

Garlic (Allium sativum L.) is widely used in various food products and traditional medicine. Besides unique taste and flavour, it is well known for its chemical profile and bioactive potential. The aim of this study was to apply subcritical water extraction (SWE) and pressurized liquid extraction (PLE) for the extraction of bioactive compounds from the Ranco genotype of garlic. Moreover, PLE process was optimized using response surface methodology (RSM) in order to determine effects and optimize ethanol concentration (45–75%), number of cycles (1–3), extraction time (1–3 min) and temperature (70–110 C) for maximized total phenols content (TP) and antioxidant activity evaluated by various in vitro assays. Furthermore, temperature effect in SWE process on all responses was evaluated, while allicin content (AC), as a major organosulphur compound, was determined in all samples. Results indicated that PLE provided tremendous advantage over SWE in terms of improved yield and antioxidant activity of garlic extracts. Therefore, high-pressure processes could be used as clean and green procedures for the isolation of garlic bioactives.

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Keywords

garlic, pressurized liquid extraction, subcritical water extraction, polyphenols, allicin

Citation

Krstić, M.; Teslić, N.; Bošković, P.; Obradović, D.; Zeković, Z.; Milić, A.; Pavlić, B. Isolation of Garlic Bioactives by Pressurized Liquid and SubcriticalWater Extraction. Molecules 2023, 28, 369. https://doi.org/10.3390/ molecules28010369