Ilić, NebojšaDey, MoulPoulev, Alexander A.Logendra, SithesKuhn, Peter E.Raskin, Ilya2017-04-262017-04-262014-10-29N.M. Ilić, M. Dey, A. Poulev, S. Logendra, P. Kuhn, I. Raskin. Anti-inflammatory activity of grains of paradise (Aframomum melegueta Schum) extract. Journal of Agricultural and Food Chemistry, 62, 43 (2014) 10452–10457. DOI: 10.1021/jf50260860021-8561http://oa.fins.uns.ac.rs/handle/123456789/14peer-reviewedThe ethanolic extract of grains of paradise (Aframomum melegueta Schum, Zingiberaceae) has been evaluated for inhibitory activity on cyclooxygenase-2 (COX-2) enzyme, in vivo for the anti-inflammatory activity and expression of several pro-inflammatory genes. Bioactivity-guided fractionation showed that the most active COX-2 inhibitory compound in the extract was [6]-paradol. [6]-Shogaol, another compound from the extract, was the most active inhibitory compound in pro-inflammatory gene expression assays. In a rat paw edema model, the whole extract reduced inflammation by 49% at 1000 mg/kg. Major gingerols from the extract [6]-paradol, [6]-gingerol, and [6]-shogaol reduced inflammation by 20, 25 and 38%. respectively when administered individually at a dose of 150 mg/kg. [6]-Shogaol efficacy was at the level of aspirin, used as a positive control. Grains of paradise extract has demonstrated an anti-inflammatory activity, which is in part due to the inhibition of COX-2 enzyme activity and expression of pro-inflammatory genes.enAframomum melegueta Schumanti-inflammatoryCOX-2gingerolsgrains of paradisepaw edemaZingiberaceaeinfo:eu-repo/semantics/article