Tomato waste: Carotenoids content, antioxidant and cell growth activities
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Date
2015-04-01
Authors
Stajčić, Slađana
Ćetković, Gordana
Čanadanović-Brunet, Jasna
Đilas, Sonja
Mandić, Anamarija 
Četojević-Simin, Dragana
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The carotenoid content, antioxidant and cell growth activities of tomato waste extracts, obtained from five different tomato genotypes, was investigated. High performance liquid chromatography was used to identify and quantify the main carotenoids present in tomato waste extracts. The antioxidant activity of tomato waste extracts was tested using spectrophotometric methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power assay. The highest DPPH scavenging activity (IC50 = 0.057 mg/ml) was obtained for Bačka extract. The Knjaz extract showed the best reducing power (IC50 = 2.12 mg/ml). Cell growth effects were determined in HeLa, MCF7 and MRC-5 cell lines by sulforhodamine B test. Anti-proliferative effects were observed in all cell lines at higher concentrations (⩾0.125 mg/ml). The carotenoid contents exhibited a strong correlation with antioxidant and anti-proliferation activity. The results obtained indicated that tomato waste should be regarded as potential nutraceutic resource and may be used as a functional food ingredient.
Description
peer-reviewed
Keywords
tomato waste, carotenoids, HPLC/DAD, reducing power, DPPH assay, cell growth activity
Citation
S. Stajčić, G. Ćetković, J. Čanadanović-Brunet, S. Đjilas, A. Mandić, D. Četojević-Simin. Tomato waste: Carotenoids content, antioxidant and cell growth activities. Food Chemistry 172 (2015) 225–232. DOI: 10.1016/j.foodchem.2014.09.069