Inulin Determination by an Improved HPLC-ELSD Method
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Date
2022
Authors
Perović, Jelena
Kojić, Jovana 
Krulj, Jelena
Pezo, Lato
Tumbas Šaponjac, Vesna
Ilić, Nebojša
Bodroža Solarov, Marija
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Since inulin utilization as a sugar or fat replacer, texture modifer, and emulsifer is increasing in the food industry, there is a need for its determination for food control and labeling. This study reports the development and validation of an advanced
high-performance liquid chromatographic method with evaporative light-scattering detection (HPLC-ELSD) method for
inulin quantifcation, with optimized extraction conditions (1:10 w/v solid-to-solvent ratio, 1 h/80 °C in a water bath continued with 30 min ultrasonication for the extraction step, and precipitation during the night with four volumes of acetone). The rapid HPLC-ELSD analysis (16 min) has been performed by isocratic elution with water as a mobile phase and optimized detector parameters (temperature of evaporator 80 °C, temperature of nebuliser 80 °C, gas fow rate 1.3 standard liters per minute, detector gain 1). The method was validated in terms of limits of detection and quantifcation, intra- and inter-day precision and accuracy, and recovery. The proposed method was carried out using a cation exchange (Ag+) column for the frst time for the inulin determination, avoiding the hydrolysis step in order to simplify and accelerate the quantifcation.
Description
Keywords
Inulin, HPLC-ELSD, Cation exchange column, Cation exchange column, Method development, Validation