Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine

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dc.contributor.authorJovanov, Pavle
dc.contributor.authorVraneš, Milan
dc.contributor.authorSakač, Marijana
dc.contributor.authorGadžurić, Slobodan
dc.contributor.authorPanić, Jovana
dc.contributor.authorMarić, Aleksandar
dc.contributor.authorOstojić, Sergej
dc.dateinfo:eu-repo/date/embargoEnd/2019-11-01
dc.date.accessioned2018-04-23T13:39:03Z
dc.date.available2018-04-23T13:39:03Z
dc.date.issued2018
dc.descriptionpeer-revieweden_US
dc.description.abstractThe biosynthesis of creatine (Cr) is closely related to the bioavailability of guanidinoacetate (GAA). The lack of one or the other may compromise their role in the energy transport and cell signaling. A reliable estimate of their levels in biological samples is imperative since they are important markers of many metabolic disorders. Therefore, a new LC-MS/MS method for simultaneous determination and quantification of GAA and Cr by multiple reaction monitoring (MRM) mode was developed based on the hydrophilic interaction chromatography (HILIC) and response surface methodology (RSM) for the optimization of chromatographic parameters. The optimized parameters ensured good separation of these similar, very polar molecules (chromatographic resolution > 1.5) without prior derivatization step in a short analysis run (6 min). The developed method was validated to ensure accurate (R, 75.1–101.6%), precise (RSD < 20%) and low quantification (LOQ of 0.025 μg mL-1 for GAA and 0.006 μg mL-1 for Cr) of the tested analytes and the use of matrix-matched calibration eliminated variable effects of complex matrices such as human plasma and urine. Therefore, this method can be implemented in medical laboratories as a tool for the diagnostics of creatine deficiencies and monitoring of guanidinoacetate and creatine supplementation regimes in biological samples.en_US
dc.description.sponsorshipThe authors appreciate funding’s from the Ministry of Education and Science (Project No. ON172012 and TR31029) of the Republic of Serbia, Provincial Secretariat for Higher Education and Scientific Research of the Autonomous Province of Vojvodina (Project No. 114-451-2379/2016-03) and European Union’s Horizon 2020 Spreading Excellence and Widening Participation programme (grant agreement No.692276, project FOODstars: Innovative Food Product Development Cycle - Frame of Stepping Up Research Excellence of FINS).en_US
dc.identifier.citationP., Jovanov, M., Vraneš, M., Sakač, S., Gadžurić, J., Panić, A., Marić, S., Ostojić. (2018) Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine. Analytica Chimica Acta, 1028, 96-103. DOI: 10.1016/j.aca.2018.03.038en_US
dc.identifier.issn0003-2670
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/70
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/692276/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31029/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172012/RS//
dc.relation.ispartofseries001;0065
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectcreatineen_US
dc.subjectguanidinoacetateen_US
dc.subjectLC-MS/MSen_US
dc.subjectHILICen_US
dc.subjectRSMen_US
dc.titleHydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatineen_US
dc.title.alternative-en_US
dc.typeinfo:eu-repo/semantics/articleen_US

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