Extraction methods of Amaranthus sp. grain oil isolation.
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Date
2016-08-15
Authors
Krulj, Jelena
Brlek, Tea
Pezo, Lato
Brkljača, Jovana
Popović, Sanja
Zeković Zoran
Bodroža Solarov, Marija 
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons Ltd.
Abstract
BACKGROUND
Amaranthus sp. is a fast-growing crop with well-known beneficial nutritional values (rich in protein, fat, dietary fiber, ash, and minerals, especially calcium and sodium, and containing a higher amount of lysine than conventional cereals). Amaranthus sp. is an underexploited plant source of squalene, a compound of high importance in the food, cosmetic and pharmaceutical industries.
RESULTS
This paper has examined the effects of the different extraction methods (Soxhlet, supercritical fluid and accelerated solvent extraction) on the oil and squalene yield of three genotypes of Amaranthus sp. grain. The highest yield of the extracted oil (78.1 g kg−1) and squalene (4.7 g kg−1) in grain was obtained by accelerated solvent extraction (ASE) in genotype 16. Post hoc Tukey's HSD test at 95% confidence limit showed significant differences between observed samples. Principal component analysis (PCA) and cluster analysis (CA) were used for assessing the effect of different genotypes and extraction methods on oil and squalene yield, and also the fatty acid composition profile. Using coupled PCA and CA of observed samples, possible directions for improving the quality of product can be realized.
CONCLUSION
The results of this study indicate that it is very important to choose both the right genotype and the right method of extraction for optimal oil and squalene yield. © 2015 Society of Chemical Industry
Description
peer-reviewed
Keywords
amaranthus sp., extraction methods, fatty acid, oil yield, squalene
Citation
Krulj, J., Brlek, T., Pezo, L., Brkljača, J., Popović, S., Zeković, Z., Bodroža Solarov, M. (2016) Extraction methods of Amaranthus sp. grain oil isolation. Journal of the Science of Food and Agriculture, 96, 10, 3352–3558. DOI: 10.1002/jsfa.7540