Simultaneous Hydrolysis of Ellagitannins and Extraction of Ellagic Acid from Defatted Raspberry Seeds Using Natural Deep Eutectic Solvents (NADES)

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dc.contributor.authorTeslić, Nemanja
dc.contributor.authorSantos, Filipa
dc.contributor.authorStupar, Alena
dc.contributor.authorPojić, Milica
dc.contributor.authorMandić, Anamarija
dc.contributor.authorPavlić, Branimir
dc.contributor.authorCvetanović Kljakić, Aleksandra
dc.contributor.authorDuarte, Ana Rita C.
dc.contributor.authorPaiva, Alexandre
dc.contributor.authorMišan, Aleksandra
dc.date.accessioned2023-07-25T10:07:28Z
dc.date.available2023-07-25T10:07:28Z
dc.date.issued2022-01-28
dc.description.abstractDefatted raspberry seeds were used as an alternative source of antioxidants and ellagic acid (EA) extracted using Natural deep eutectic solvents (NADES). In the preliminary study, the best NADES combination (citric acid-betaine) and the most influential variables (temperature, time, and NADES/plant ratio) were selected for the further optimization process. All samples were analyzed in terms of total polyphenol, EA content, and antioxidant activity. Two sets of optimal conditions were generated by response surface methodology. The first set (Opt1) was designed for higher conversion of ellagitannins to EA while the latter set (Opt2) for higher EA content/100 g extract. Opt1 and Opt2 had higher values for all investigated responses compared to 80% ethanolic extract but had a lower conversion rate of ellagitannins to EA compared to acidified methanol extract. The third set of parameters (Opt3) selected beyond the initial experimental domain was used to obtain a sample with the highest EA content/100 g extract. Due to their nature, NADES extracts are ready to use and could have various technological roles in products since they are antioxidants, acidifiers, and colorants. NADES raspberry extracts exhibited higher anti-proliferative activity compared to ethanolic extracts in terms of EC50 values. However, the main contributor of anti-cancer activity in NADES raspberry extracts were individual NADES compounds and/or their newly formed NADES structure.en_US
dc.description.sponsorshipAuthors would like to thank the Ministry of Education, Science and Technological Development of the Republic of Serbia, Grant No. 451-03-9/2021-14/ 200222; the Science Fund of the Republic of Serbia, PROMIS, Grant No. 6060592, DEStiny; and the European Union, Horizon 2020 (European Research Council), Grant no ERC-2016-CoG 72503 for the funding.en_US
dc.identifier.citationTeslić, N.; Santos, F.; Oliveira, F.; Stupar, A.; Pojić, M.; Mandić, A.; Pavlić, B.; Kljakić, A.C.; Duarte, A.R.C.; Paiva, A.; et al. Simultaneous Hydrolysis of Ellagitannins and Extraction of Ellagic Acid from Defatted Raspberry Seeds Using Natural Deep Eutectic Solvents (NADES). Antioxidants 2022, 11, 254. https://doi.org/10.3390/antiox11020254en_US
dc.identifier.doi10.3390/antiox11020254
dc.identifier.issn2076-3921
dc.identifier.scopus2-s2.0-85123547890
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/337
dc.identifier.wos000762505000001
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relationinfo:eu-repo/grantAgreement/ScienceFundRS/Promis/6060592/RS//
dc.rightsopenAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectellagic aciden_US
dc.subjecthydrolysis and extractionen_US
dc.subjectby-product valorizationen_US
dc.subjectacidic NADESen_US
dc.titleSimultaneous Hydrolysis of Ellagitannins and Extraction of Ellagic Acid from Defatted Raspberry Seeds Using Natural Deep Eutectic Solvents (NADES)en_US
dc.typeinfo:eu-repo/semantics/article

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