Valorization of raspberry seeds using acidic NADES: A preliminary study

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dc.contributor.authorTeslić, Nemanja
dc.contributor.authorOliveira, Filipe
dc.contributor.authorSantos, Filipa
dc.contributor.authorStupar, Alena
dc.contributor.authorPojić, Milica
dc.contributor.authorMandić, Anamarija
dc.contributor.authorPavlić, Branimir
dc.contributor.authorCvetanović, Aleksandra
dc.contributor.authorDuarte, Ana Rita C.
dc.contributor.authorMišan, Aleksandra
dc.date.accessioned2023-07-27T06:41:56Z
dc.date.available2023-07-27T06:41:56Z
dc.date.issued2022-09
dc.description.abstractBy-products of raspberry seeds fruit processing industry are rich in bioactive compounds. Ellagic acid (EA) is a polyphenol naturally present in raspberry seeds which has antioxidant, hepatoprotective, antimicrobial, anti-carcinogenic, antiinflammatory activity, among other interesting bioactivities. EA majority is bonded in the form of ellagitannins (ETs) which have lower bioactivity when comparing to EA. Hence, ETs have to be hydrolysed to EA. Classical acid hydrolysis of ETs requires utilization of mineral acids (e.g. HCl) and alcohol (e.g. MeOH) which are detrimental for human health and hazardous for the environment. Natural Deep Eutectic Systems (NADES) composed of e.g. citric acid and betaine could serve as alternative medium for the hydrolysis process. This would allow to production edible, non-toxic and ready-to-use extracts which could be further used in food and cosmetic industry. Hydrolysis of ETs to EA using NADES is determined by numerous parameters (e.g. temperature, time, molar ratio of citric acid and betaine in NADES, water content in NADES, NADES/plant ratio etc.) and process optimization with all variables could be time consuming, inefficient and expensive. Thus, it is necessary to reduce their number and determine the most influential variables prior to final optimization step. For that purpose, 25-1 factorial experimental design was applied with a total of 16 experimental setups. The obtained extracts were analysed in terms of EA content which was determined by HPLC method, total polyphenol content analysed with Folin-Ciocalteu procedure and in vitro antioxidant activity determined by DPPH assay. Among all selected variables, NADES/plant ratio, temperature, and time, exhibited the highest impact on EA content, thus, these parameters should be selected for the final optimization step.en_US
dc.description.sponsorshipAuthors would like to thank the Ministry of education, science and technological development of the Republic of Serbia, Grant No. 451-03-9/2021-14/ 200222; the Science Fund of the Republic of Serbia, PROMIS, Grant No. 6060592, DEStiny; and the European Union, Horizon 2020 (European Research Council), Grant no ERC-2016-CoG 72503 for the funding.en_US
dc.identifier.citationTeslić, N., Oliveira, F., Santos, F., Stupar, A., Pojić, M., Mandić, A., Pavlić, B., Cvetanović A., Duarte, A.R.C., Mišan, A. (2022) Valorization of raspberry seeds using acidic NADES: A preliminary study. 4th Edition of Euro-Global Conference on Food Science and Technology, 12-13. September, Book of Abstracts 51.en_US
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/354
dc.language.isoenen_US
dc.publisherMagnus groupen_US
dc.relationinfo:eu-repo/grantAgreement/ScienceFundRS/Promis/6060592/RS//
dc.rightsopenAccess
dc.titleValorization of raspberry seeds using acidic NADES: A preliminary studyen_US
dc.typeinfo:eu-repo/semantics/otheren_US

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