Green methods for protein isolation: sustainable approaches for enhanced protein recovery

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dc.contributor.authorMarić, Aleksandar
dc.contributor.authorŠarić, Bojana
dc.contributor.authorSakač, Marijana
dc.contributor.authorĐermanović, Branislava
dc.contributor.authorKojić, Jovana
dc.contributor.authorŠarić, Ljubiša
dc.contributor.authorJovanov, Pavle
dc.date.accessioned2023-09-25T08:55:21Z
dc.date.available2023-09-25T08:55:21Z
dc.date.issued2023-09
dc.description.abstractProtein isolation is a crucial step in obtaining pure and functional proteins for various applications. Traditional protein isolation methods often involve the use of harsh chemicals, high energy consumption, and generate significant amounts of waste. In contrast, green methods employ environmentally friendly techniques that minimize the use of hazardous chemicals, conserve energy, and promote the efficient utilization of resources. Several innovative green approaches for protein isolation have emerged, including enzyme-assisted extraction, aqueous two-phase systems, and membrane-based separation techniques. These methods offer numerous advantages such as higher selectivity, improved protein recovery, and reduced environmental footprint. Enzyme-assisted extraction utilizes proteolytic enzymes to selectively cleave proteins from their matrix, enabling gentle and efficient protein isolation. Aqueous two-phase systems exploit the phase separation of two immiscible aqueous phases to partition proteins based on their physicochemical properties, facilitating their separation. Membrane-based separation techniques employ membranes with specific pore sizes or affinity ligands to selectively isolate proteins from complex mixtures. By adopting green methods, researchers and industries can contribute to sustainable protein production, reduce waste generation, and minimize the use of hazardous chemicals. Additionally, the recovered proteins can be used in various applications, including food, pharmaceuticals, and biotechnology, ensuring their valuable utilization.en_US
dc.description.sponsorshipThis work was financially supported by the Ministry of Science, Technological Development and Innovations, Republic of Serbia (Contract No. 451-03-47/2023-01/200222), as well as by the Science Fund of the Republic of Serbia, Grant No 6673, PROTein from Rapeseed Oil Processing Waste: Application in Food and Wastewater Treatment – PROTOPOWER.en_US
dc.identifier.citationMarić, A., Šarić, B., Sakač, M., Đermanović, B., Kojić, J., Šarić, Lj., Jovanov, P. (2023). Green methods for protein isolation: sustainable approaches for enhanced protein recovery. 14th International Scientific and Professional Conference WITH FOOD TO HEALTH, Osijek, Croatia, 14–15 September, 2023, 54.en_US
dc.identifier.isbn978-953-7005-92-4
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/398
dc.language.isoenen_US
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200222/RS// info:eu-repo/grantAgreement/ScienceFundRS/Zeleni/6673/RS//
dc.subjectprotein isolationen_US
dc.subjectgreen methodsen_US
dc.subjectenzyme-assisted extractionen_US
dc.subjectaqueous two-phase systemsen_US
dc.subjectmembrane-based separation techniquesen_US
dc.titleGreen methods for protein isolation: sustainable approaches for enhanced protein recoveryen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US

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