UHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extracts

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dc.contributor.authorMarić, Boško
dc.contributor.authorAbramović, Biljana
dc.contributor.authorIlić, Nebojša
dc.contributor.authorBodroža Solarov, Marija
dc.contributor.authorPavlić, Branimir
dc.contributor.authorOczkowski, Michał
dc.contributor.authorWilczak, Jacek
dc.contributor.authorČetojević-Simin, Dragana
dc.contributor.authorŠarić, Ljubiša
dc.contributor.authorTeslić, Nemanja
dc.date.accessioned2023-07-26T08:14:19Z
dc.date.available2023-07-26T08:14:19Z
dc.date.issued2022-12-28
dc.description.abstractThe primary aim of this experiment was to investigate the bioactivity potential and polyphenolic profile of defatted raspberry seeds (DRS) extracts from three varieties (Willamette, Meeker, and Polka) using the in vitro tests HPLC-DAD and UHPLC-Triple-TOF-MS. Extracts were obtained using ultrasound-assisted extraction (UAE) or hydrolysis. The antioxidant activity of the extracts was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonic) cation (ABTS), and ferric reducing antioxidant power (FRAP) assays. Furthermore, the extracts were tested for antimicrobial activity using the disk diffusion method for four bacterial cultures (Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella enterica subsp. enterica Enteritidis). In vitro antiproliferative activity was tested using cervical carcinoma (HeLa), breast adenocarcinoma (MCF7), and fetal lung (MRC-5) human cell lines. In total, 32 phenolic compounds were detected in DRS extracts. A small quantity of ellagic acid (EA) was in free form, while EA content increased after the hydrolysis process. The extracts from the Meeker variety exhibited the highest antioxidant activity, analyzed with DPPH and FRAP assays, while extracts from the Polka variety had the highest activity towards ABTS + radical scavenging activity. The UAE samples expressed higher antiproliferative activity in comparison to hydrolysis extracts. The results indicate that DRS extracts have certain bioactivity, and their use in the food, cosmetic, and pharmaceutical industries is recommended.en_US
dc.description.sponsorshipThis research was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia and is gratefully acknowledged (Grant No. 451-03-68/2022-14/200222).en_US
dc.identifier.citationMarić, B.; Abramović, B.; Ilić, N.; Bodroža-Solarov, M.; Pavlić, B.; Oczkowski, M.;Wilczak, J.; Četojević-Simin, D.; Šarić, L.; Teslić, N. UHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extracts. Foods 2023, 12, 161. https://doi.org/10.3390/ foods12010161en_US
dc.identifier.doi10.3390/foods12010161
dc.identifier.issn2304-8158
dc.identifier.scopus2-s2.0-85145947426
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/344
dc.identifier.wos000909116600001
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200222/RS//
dc.rightsopenAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbioactivityen_US
dc.subjectraspberry seedsen_US
dc.subjectpolyphenols profileen_US
dc.subjectantioxidant activityen_US
dc.subjectantiproliferative activityen_US
dc.titleUHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extractsen_US
dc.typeinfo:eu-repo/semantics/articleen_US

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