POLYPHENOLS PROFILE OF WILD THYME EXTRACTS OBTAINED BY CONVENTIONAL SOLID-LIQUID AND ULTRASOUND-ASSISTED EXTRACTION

dc.contributor.authorMrkonjić, Živan
dc.contributor.authorRakić, Dušan
dc.contributor.authorKaplan, Muammer
dc.contributor.authorTeslić, Nemanja
dc.contributor.authorZeković, Zoran
dc.contributor.authorLazarević, Ivana
dc.contributor.authorPavlić, Branimir
dc.date.accessioned2023-07-27T08:28:56Z
dc.date.available2023-07-27T08:28:56Z
dc.date.issued2022-10
dc.description.abstractThymus serpyllum extracts, potentially enriched with a wide spectrum of polyphenols, were subjected to LC-MS-MS analysis in order to determine their qualitative polyphenols profile. Analyses were conducted in the sample with the highest polyphenols yield and antioxidant activity obtained by conventional extraction using 60% of ethanol for 24 h at shaking speed of 150 rpm (Sample CE), sample obtained by ultrasound-assisted extraction at 65 °C for 55 min using 60% ethanol (Sample UAE-CP) and sample obtained at the temperature of 70.28 °C, extraction time of 70 min and using 45% ethanol as a solvent (Sample UAE-OPT). According to results of identification, the Sample UAE-CP contained the highest number of compounds, 31, a total of 30 different compounds were identified in the Sample CE and 27 compounds in the Sample UAE-OPT. Identified flavonoid subgroups were flavanones, flavan-3-ols, flavonols, flavones and isoflavones. Some of them were identified in aglycone form only. On the other side, flavanones were identified not only in aglycone form, but also in a form of their 7-O-glucoside, e.g. naringenin-7-O-glucoside. Finally, flavonols were identified in various forms, as compounds in aglycone form, then in form of their 3-O-glycosides with rutinose, glucose and galactose as the carbohydrate compounds, but in form of glucuronide and hexoside isomer as well. Identified phenolic acids were gallic, vanillic, ellagic, caffeic, 3-p-coumaroylquinic, 4-p-coumaroylquinic acid, coumaric acid hexoside isomer, p-coumaric, cis- and trans-coutaric acid. Taking into account the discrepancy in the polyphenols profiles of aforementioned extracts, it could be concluded that different extraction techniques under different extraction conditions or using different extraction solvent could be of a great importance for establishing the adequate polyphenols profile of T. serpyllum extracts.en_US
dc.description.sponsorshipThis research was supported by the Science Fund of the Republic of Serbia, [7750168], Novel extracts and bioactive compounds from under-utilized resources for high-value applications— BioUtilize.en_US
dc.identifier.citationMrkonjić, Ž., Rakić, D., Kaplan, M., Teslić, N., Zeković, Z., Lazarević, I., Pavlić, B. (2022) POLYPHENOLS PROFILE OF WILD THYME EXTRACTS OBTAINED BY CONVENTIONAL SOLID-LIQUID AND ULTRASOUND-ASSISTED EXTRACTION. 2nd International Conference on Advanced Production and Processing, 20-22. October, Novi Sad, Serbia, Book of Abstracts, 162.en_US
dc.identifier.isbn978-86-6253-160-5
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/357
dc.language.isoenen_US
dc.publisherPublisher: University of Novi Sad, Faculty of Technology Novi Saden_US
dc.relationinfo:eu-repo/grantAgreement/ScienceFundRS/Ideje/7750168/RS//
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectThymus serpyllumen_US
dc.subjectHerbal dusten_US
dc.subjectBy-producten_US
dc.subjectAntioxidantsen_US
dc.titlePOLYPHENOLS PROFILE OF WILD THYME EXTRACTS OBTAINED BY CONVENTIONAL SOLID-LIQUID AND ULTRASOUND-ASSISTED EXTRACTIONen_US
dc.typeinfo:eu-repo/semantics/otheren_US

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