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Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine

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dc.contributor.author Jovanov, Pavle
dc.contributor.author Vraneš, Milan
dc.contributor.author Sakač, Marijana
dc.contributor.author Gadžurić, Slobodan
dc.contributor.author Panić, Jovana
dc.contributor.author Marić, Aleksandar
dc.contributor.author Ostojić, Sergej
dc.date info:eu-repo/date/embargoEnd/2019-11-01
dc.date.accessioned 2018-04-23T13:39:03Z
dc.date.available 2018-04-23T13:39:03Z
dc.date.issued 2018
dc.identifier.citation P., Jovanov, M., Vraneš, M., Sakač, S., Gadžurić, J., Panić, A., Marić, S., Ostojić. (2018) Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine. Analytica Chimica Acta, 1028, 96-103. DOI: 10.1016/j.aca.2018.03.038 en_US
dc.identifier.issn 0003-2670
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/70
dc.description peer-reviewed en_US
dc.description.abstract The biosynthesis of creatine (Cr) is closely related to the bioavailability of guanidinoacetate (GAA). The lack of one or the other may compromise their role in the energy transport and cell signaling. A reliable estimate of their levels in biological samples is imperative since they are important markers of many metabolic disorders. Therefore, a new LC-MS/MS method for simultaneous determination and quantification of GAA and Cr by multiple reaction monitoring (MRM) mode was developed based on the hydrophilic interaction chromatography (HILIC) and response surface methodology (RSM) for the optimization of chromatographic parameters. The optimized parameters ensured good separation of these similar, very polar molecules (chromatographic resolution > 1.5) without prior derivatization step in a short analysis run (6 min). The developed method was validated to ensure accurate (R, 75.1–101.6%), precise (RSD < 20%) and low quantification (LOQ of 0.025 μg mL-1 for GAA and 0.006 μg mL-1 for Cr) of the tested analytes and the use of matrix-matched calibration eliminated variable effects of complex matrices such as human plasma and urine. Therefore, this method can be implemented in medical laboratories as a tool for the diagnostics of creatine deficiencies and monitoring of guanidinoacetate and creatine supplementation regimes in biological samples. en_US
dc.description.sponsorship The authors appreciate funding’s from the Ministry of Education and Science (Project No. ON172012 and TR31029) of the Republic of Serbia, Provincial Secretariat for Higher Education and Scientific Research of the Autonomous Province of Vojvodina (Project No. 114-451-2379/2016-03) and European Union’s Horizon 2020 Spreading Excellence and Widening Participation programme (grant agreement No.692276, project FOODstars: Innovative Food Product Development Cycle - Frame of Stepping Up Research Excellence of FINS). en_US
dc.language.iso en en_US
dc.publisher ELSEVIER en_US
dc.relation info:eu-repo/grantAgreement/EC/H2020/692276/EU//
dc.relation info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31029/RS//
dc.relation info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172012/RS//
dc.relation.ispartofseries 001;0065
dc.rights info:eu-repo/semantics/embargoedAccess
dc.subject creatine en_US
dc.subject guanidinoacetate en_US
dc.subject LC-MS/MS en_US
dc.subject HILIC en_US
dc.subject RSM en_US
dc.title Hydrophilic interaction chromatography coupled to tandem mass spectrometry as a method for simultaneous determination of guanidinoacetate and creatine en_US
dc.title.alternative - en_US
dc.type info:eu-repo/semantics/article en_US


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