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Isolation of Garlic Bioactives by Pressurized Liquid and SubcriticalWater Extraction

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dc.contributor.author Krstić, Marko
dc.contributor.author Teslić, Nemanja
dc.contributor.author Bošković, Perica
dc.contributor.author Obradović, Darija
dc.contributor.author Zeković, Zoran
dc.contributor.author Milić, Anita
dc.contributor.author Pavlić, Branimir
dc.date.accessioned 2023-07-26T12:27:08Z
dc.date.available 2023-07-26T12:27:08Z
dc.date.issued 2023-01-02
dc.identifier.citation Krstić, M.; Teslić, N.; Bošković, P.; Obradović, D.; Zeković, Z.; Milić, A.; Pavlić, B. Isolation of Garlic Bioactives by Pressurized Liquid and SubcriticalWater Extraction. Molecules 2023, 28, 369. https://doi.org/10.3390/ molecules28010369 en_US
dc.identifier.issn 1420-3049
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/348
dc.description.abstract Garlic (Allium sativum L.) is widely used in various food products and traditional medicine. Besides unique taste and flavour, it is well known for its chemical profile and bioactive potential. The aim of this study was to apply subcritical water extraction (SWE) and pressurized liquid extraction (PLE) for the extraction of bioactive compounds from the Ranco genotype of garlic. Moreover, PLE process was optimized using response surface methodology (RSM) in order to determine effects and optimize ethanol concentration (45–75%), number of cycles (1–3), extraction time (1–3 min) and temperature (70–110 C) for maximized total phenols content (TP) and antioxidant activity evaluated by various in vitro assays. Furthermore, temperature effect in SWE process on all responses was evaluated, while allicin content (AC), as a major organosulphur compound, was determined in all samples. Results indicated that PLE provided tremendous advantage over SWE in terms of improved yield and antioxidant activity of garlic extracts. Therefore, high-pressure processes could be used as clean and green procedures for the isolation of garlic bioactives. en_US
dc.description.sponsorship This research was supported by the Science Fund of the Republic of Serbia, 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications— BioUtilize. en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.relation info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7750168/RS//
dc.rights openAccess
dc.rights info:eu-repo/semantics/openAccess
dc.subject garlic en_US
dc.subject pressurized liquid extraction en_US
dc.subject subcritical water extraction en_US
dc.subject polyphenols en_US
dc.subject allicin en_US
dc.title Isolation of Garlic Bioactives by Pressurized Liquid and SubcriticalWater Extraction en_US
dc.type info:eu-repo/semantics/article en_US
dc.identifier.scopus 2-s2.0-85145693706
dc.identifier.wos 000909249400001
dc.identifier.doi 10.3390/molecules28010369


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