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Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts

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dc.contributor.author Pavlić, Branimir
dc.contributor.author Mrkonjić, Živan
dc.contributor.author Teslić, Nemanja
dc.contributor.author Cvetanović Kljakić, Aleksandra
dc.contributor.author Pojić, Milica
dc.contributor.author Mandić, Anamarija
dc.contributor.author Stupar, Alena
dc.contributor.author Santos, Filipa
dc.contributor.author Duarte, Ana Rita C.
dc.contributor.author Mišan, Aleksandra
dc.date.accessioned 2023-07-26T12:17:15Z
dc.date.available 2023-07-26T12:17:15Z
dc.date.issued 2022-02-23
dc.identifier.citation Pavlić, B.; Mrkonjić, Ž.; Teslić, N.; Kljakić, A.C.; Pojić, M.; Mandić, A.; Stupar, A.; Santos, F.; Duarte, A.R.C.; Mišan, A. Natural Deep Eutectic Solvents (NADES) Extraction Improves Polyphenols Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts. Molecules 2022, 27, 1508. https://doi.org/10.3390/ molecules27051508 en_US
dc.identifier.issn 1420-3049
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/347
dc.description.abstract Wild thyme (Thymus serpyllum L.) herbal dust has been recognized as a potential underutilized resource for the recovery of antioxidants. The aim of this paper was to optimize natural deep eutectic solvent (NADES) extraction of polyphenols to obtain improved antioxidant activity of extracts determined by selected in vitro assays (DPPH, FRAP, and ABTS). Twenty different NADES systems were investigated in the first step of the screening of the extraction solvent and L-proline (Pro)–glycerine (Gly) based solvents provided the best results. Preliminary experiments organized by 25−1 fractional factorial design narrowed down the number of extraction factors from five (temperature, extraction time, NADES type, water content and L/S ratio) to three and determined their experimental domain for the final step. A face-centered central composite design with temperature (40–55–70 °C), extraction time (60–120–180 min) and L/S ratio (10–20–30 g NADES/g sample) was applied for influence analysis and process optimization. Multi-response optimization suggested a temperature of 65 °C, time of extraction of 180 min and L/S ratio of 28 g NADES/g DW as optimal extraction parameters. Experimental validation confirmed good agreement between experimental and predicted results in the extract obtained at optimal conditions and the interactions in the most suitable NADES (N16; Pro–Gly–H2O; 1:2:1) were confirmed by the 1H-NMR. en_US
dc.description.sponsorship This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions—DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications— BioUtilize. This project received funding from the European Union’s Horizon 2020—European Research Council (ERC)—under grant agreement No ERC-2016-CoG 725034. This work was supported by the Associate Laboratory for Green Chemistry (LAQV), which is financed by national funds from FCT/MCTES (UIDB/50006/2020). en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.relation info:eu-repo/grantAgreement/ScienceFundRS/Promis/6060592/RS//
dc.rights openAccess
dc.rights info:eu-repo/semantics/openAccess
dc.subject Thymus serpyllum L. en_US
dc.subject NADES en_US
dc.subject polyphenols en_US
dc.subject antioxidant activity en_US
dc.subject RSM optimization en_US
dc.title Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts en_US
dc.type info:eu-repo/semantics/article en_US
dc.identifier.scopus 2-s2.0-85125173144
dc.identifier.wos 000775091300001
dc.identifier.doi 10.3390/molecules27051508


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