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Chemical Composition, Antimicrobial Activity, and Withdrawal Period of Essential Oil-Based Pharmaceutical Formulation in Bovine Mastitis Treatment

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dc.contributor.author Kovačević, Zorana
dc.contributor.author Tomanić, Dragana
dc.contributor.author Čabarkapa, Ivana
dc.contributor.author Šarić, Ljubiša
dc.contributor.author Stanojević, Jovan
dc.contributor.author Bijelić, Katarina
dc.contributor.author Galić, Ivan
dc.contributor.author Ružić, Zoran
dc.contributor.author Erdeljan, Mihajlo
dc.contributor.author Kladar, Nebojša
dc.date.accessioned 2023-07-14T09:46:29Z
dc.date.available 2023-07-14T09:46:29Z
dc.date.issued 2022-12-07
dc.identifier.citation 3. Kovačević, Z.; Tomanić, D.; Čabarkapa, I.; Šarić, Lj.; Stanojević, J.; Bijelić, K.; Galić, I.; Ružić, Z.; Erdeljan, M.; and Kladar, N. Chemical composition, Antimicrobial activity and Withdrawal period of Essential Oils Based Pharmaceutical Formulation in Bovine Mastitis Treatment, Int. J. Environ. Res. Public Health. (2022), 19(24), 16643; https://doi.org/10.3390/ijerph192416643 en_US
dc.identifier.issn 1660-4601
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/263
dc.description.abstract Due to the emergence of antibiotic-resistant bacteria, the risk it represents to public health, and the possible consequences for animal health and welfare, there is an increasing focus on reducing antimicrobial usage (AMU) in animal husbandry. Therefore, a great interest in developing alternatives to AMU in livestock production is present worldwide. Recently, essential oils (EOs) have gained great attention as promising possibilities for the replacement of antibiotics. The current study aimed to test the potential of using a novel EO-based pharmaceutical formulation (Phyto-Bomat) in bovine mastitis treatment. The antibacterial activity was performed using the microdilution technique. Lactating dairy cows were treated with 15 mL of Phyto-Bomat in the inflamed quarter for 5 consecutive days in order to analyze blood and milk samples for thymol and carvacrol residues using gas chromatography and mass spectrometry (GC–MS). Antimicrobial activity expressed as the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) indicates that this formulation has the highest activity against Gram-positive strains. The dominant compounds in Phyto-Bomat were thymol and carvacrol, at 12.58 ± 1.23 mg/mL and 23.11 ± 2.31 mg/mL, respectively. The quantification of these two compounds in evaluated biological samples showed that 24 h after administration the concentration of thymol and carvacrol in milk samples was at the same level as before application. On the other hand, thymol and carvacrol were detectable in plasma samples even after 24 h post-treatment, with values ranging from 0.15–0.38 and 0.21–0.66 µg/mL, respectively. The tested formulation showed encouraging results of antibacterial activity against bovine mastitis pathogens, as well as the withdrawal period of dominant compounds, which implies that further testing regarding the bacteriological and clinical cure rates in clinical settings is needed. en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.subject essential oils residues en_US
dc.subject withdrawal period en_US
dc.subject mastitis en_US
dc.subject antimicrobials en_US
dc.subject phyto-Bomat en_US
dc.title Chemical Composition, Antimicrobial Activity, and Withdrawal Period of Essential Oil-Based Pharmaceutical Formulation in Bovine Mastitis Treatment en_US
dc.type Article en_US
dc.type info:eu-repo/semantics/article


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