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Inulin Determination by an Improved HPLC-ELSD Method

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dc.contributor.author Perović, Jelena
dc.contributor.author Kojić, Jovana
dc.contributor.author Krulj, Jelena
dc.contributor.author Pezo, Lato
dc.contributor.author Tumbas Šaponjac, Vesna
dc.contributor.author Ilić, Nebojša
dc.contributor.author Bodroža Solarov, Marija
dc.date.accessioned 2023-08-22T13:57:05Z
dc.date.available 2023-08-22T13:57:05Z
dc.date.issued 2022
dc.identifier.issn 19369751
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/389
dc.description.abstract Since inulin utilization as a sugar or fat replacer, texture modifer, and emulsifer is increasing in the food industry, there is a need for its determination for food control and labeling. This study reports the development and validation of an advanced high-performance liquid chromatographic method with evaporative light-scattering detection (HPLC-ELSD) method for inulin quantifcation, with optimized extraction conditions (1:10 w/v solid-to-solvent ratio, 1 h/80 °C in a water bath continued with 30 min ultrasonication for the extraction step, and precipitation during the night with four volumes of acetone). The rapid HPLC-ELSD analysis (16 min) has been performed by isocratic elution with water as a mobile phase and optimized detector parameters (temperature of evaporator 80 °C, temperature of nebuliser 80 °C, gas fow rate 1.3 standard liters per minute, detector gain 1). The method was validated in terms of limits of detection and quantifcation, intra- and inter-day precision and accuracy, and recovery. The proposed method was carried out using a cation exchange (Ag+) column for the frst time for the inulin determination, avoiding the hydrolysis step in order to simplify and accelerate the quantifcation. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation info:eu-repo/grantAgreement/MESTD/inst-2020/200222/RS//
dc.rights openAccess
dc.rights info:eu-repo/semantics/openAccess
dc.subject Inulin en_US
dc.subject HPLC-ELSD en_US
dc.subject Cation exchange column en_US
dc.subject Cation exchange column en_US
dc.subject Method development en_US
dc.subject Validation en_US
dc.title Inulin Determination by an Improved HPLC-ELSD Method en_US
dc.type Article en_US
dc.type info:eu-repo/semantics/article
dc.identifier.scopus 2-s2.0-85120490823
dc.identifier.wos 000725372900001
dc.identifier.doi 10.1007/s12161-021-02140-y


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