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Antibacterial activity of donkey’s milk against clinical isolate of Klebsiella pneumoniae

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dc.contributor.author Šarić, Ljubiša
dc.contributor.author Pezo, Lato
dc.contributor.author Krulj, Jelena
dc.contributor.author Plavšić, Dragana
dc.contributor.author Jovanov, Pavle
dc.contributor.author Matić, Milana
dc.date.accessioned 2023-07-17T06:53:16Z
dc.date.available 2023-07-17T06:53:16Z
dc.date.issued 2022
dc.identifier.citation Šarić, Lj., Pezo, L., Krulj, J., Tomić, J., Plavšić, D., Jovanov, P., Matić, M. (2022). Antibacterial activity of donkey’s milk against clinical isolate of Klebsiella pneumoniae. Mljekarstvo, 72 (2), 63-76. en_US
dc.identifier.issn 1846-4025
dc.identifier.uri http://oa.fins.uns.ac.rs/handle/123456789/289
dc.description.abstract The purpose of this study was to investigate the antibacterial activity of raw donkey milk toward the clinical isolate of Klebsiella pneumoniae at 9, 15 and 38 °C as well as to clarify the role of lysozyme, lactoferrin and calcium content in the antibacterial activity of donkey’s milk. The effects of contamination level and incubation period on this antibacterial activity were also examined. Antibacterial assays were performed by determing the bacterial count in intentionally contaminated (102, 103, 104 cfu/mL) donkey milk samples during 8 and 96 hours. Lab-on-a-chip electrophoresis and atomic absorption spectrometry were used for the determination of lysozyme, lactoferrin and calcium contents in donkey milk, respectively. The donkey milk samples showed varying degrees of antibacterial activity against the tested strain K. pneumoniae. The antibacterial synergism of lysozyme and lactoferrin was proven for this clinical strain as the samples with higher lactoferrin amount showed a stronger antibacterial activity. The correlation between calcium content and antibacterial activity of donkey milk samples was not established. Donkey milk showed stronger antibacterial potential at 15 °C compared to 9 °C, but this was limited by a higher growth rate of K. pneumoniae at 15 °C. The higher level of contamination resulted in a faster consumption of the antibacterial capacity of donkey milk. The artificial neural network model for prediction of K. pneumoniae count gave acurate fit to experimental data, showing a reasonably good (overall r2 for donkey milk was 0.986, with training error 4.67∙10-4, while r2 for nutrient broth was 0.982 and training error was 0.002). en_US
dc.description.sponsorship This work was financially supported by the Provincial Secretariat for Higher Education and Scientific Research, Autonomous Province of Vojvodina, Republic of Serbia [grant number 142-451-637/2021-01/2] as a part of the project entitled "Examination of the impact of goat, mare and donkey milk on the recovery of patients with pneumonia". en_US
dc.language.iso en en_US
dc.publisher Croatian Dairy Union, Croatia en_US
dc.rights openAccess
dc.subject Donkey milk en_US
dc.subject antibacterial activity en_US
dc.subject Klebsiella pneumonia en_US
dc.title Antibacterial activity of donkey’s milk against clinical isolate of Klebsiella pneumoniae en_US
dc.type info:eu-repo/semantics/article
dc.identifier.scopus 2-s2.0-85127935404
dc.identifier.wos 000782642300001
dc.identifier.doi 10.15567/mljekarstvo.2022.0201


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